Journal: BMC Cancer

Article Title: Aqueous Cinnamon Extract (ACE- c ) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential

doi: 10.1186/1471-2407-10-210

Figure Lengend Snippet: Cinnamon reduces migration potential .(A) Photomicrographs of time-lapse image at 0 and 15 h in a wound-healing assay in cells treated with different concentrations (0-80 μg/ml) of ACE- c . The upper panel of the image shows the wound made at 0 h. The lower panel shows cell movement corresponding to the distance traveled by the cells at 15 h of time-lapse imaging. (B) Rate of migration of cells during the wound healing assay analyzed by the time-lapse imaging of SiHa cells. Migration rate (μm/h) for each sample from five different fields was calculated. Error bars represent standard deviation and the data is representative of five independent experiments. (C) ACE- c treatment reduces the MMP-2 expression at mRNA level that has been shown by RT-PCR. β-actin was used as the loading control. Densitometric analysis of MMP-2 expression was performed using phosphorimager. The data represents mean ± SD of five independent experiments. (D) Gelatin zymography showing downregulation of MMP-2 expression in SiHa cells at 80 μg/ml ACE- c treatment compared to the untreated control cells. The bands were quantified by densitometry using phosphorimager and the data represents mean ± SD of five independent experiments.

Article Snippet: The intensities of the bands corresponding to the RT-PCR products were quantified using phosphorimager (Alpha Imager using Alpha Ease FC software, Alpha Innotech) and normalized with respect to the β-actin product.

Techniques: Migration, Wound Healing Assay, Imaging, Standard Deviation, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Zymography

Journal: BMC Cancer

Article Title: Aqueous Cinnamon Extract (ACE- c ) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential

doi: 10.1186/1471-2407-10-210

Figure Lengend Snippet: Cinnamon decreased the expression of Her-2 oncoprotein . (A) Western blot analysis showing decrease in Her-2 expression in SiHa cells treated with different concentrations of ACE- c (0-80 μg/ml). Equal amounts of protein were loaded on 10% SDS-gel and immunoblotted with anti-Her-2 antibody. Tubulin was used as a loading control. Densitometric analysis of Her-2 expression was performed using phosphorimager. The data represents mean ± SD of five independent experiments. (B) Confocal images of the cells treated with indicated concentrations of ACE- c showing decrease in Her-2 expression. The cells were stained indirectly for Her-2 using Cy3 antibody (Panel II) and counterstained with DAPI (Panel I). Panel III represents the merge images.

Article Snippet: The intensities of the bands corresponding to the RT-PCR products were quantified using phosphorimager (Alpha Imager using Alpha Ease FC software, Alpha Innotech) and normalized with respect to the β-actin product.

Techniques: Expressing, Western Blot, SDS-Gel, Control, Staining